(1) At the end of the culture period cells were harvested, exposed to a hypotonic solution and fixed in methanol/acetic acid.(2) About 5 ml of hypotonic solution was added.(3) A potential limitation of the presented methods is that pressure-flow measurements were generated under hypotonic conditions.(4) All seven mutants that failed to rescue the cell from hypotonic shock did not show channel activity under patch clamping.(5) First, hypotonic treatment did not change surface adhesiveness.(6) Following hypotonic treatment and fixation, the cells are dropped on a slide and then stained.(7) However, in vivo sampling methods lead to the stimulation of a relatively hypotonic glandular secretion.(8) To collect the cells, the cultures were centrifuged and treated with hypotonic solution for 30 min at 37°C.(9) Cell harvesting and slide preparation followed standard cytogenetic procedures involving hypotonic treatment, fixation and staining.(10) On arrival, at 3 hours of age, he was hypotonic with tonic seizures of the upper extremities and bilaterally fixed, dilated pupils.(11) In IMCD cells all cellular organic osmolytes leave the cells under hypotonic conditions.(12) Vasopressin continued to act in patients excreting isotonic or hypertonic urine, so hypotonic intake must be avoided in them.(13) More commonly, hypotonic loss is seen related to gastrointestinal sources, burns, diuretic therapy or osmotic diuresis.(14) Thus, the photodamaged lysosomes and the normal ones will suffer the same level of osmotic stress in the same hypotonic sucrose medium.(15) After 15 min five drops of methanol/acetic acid were added to the hypotonic solution to begin cell fixation.(16) Cerebral palsy takes the form of a hypotonic tetraplegia when the child has a mobility problem but with floppy muscles.
