(1) Agarose gel electrophoresis was carried out in sodium barbitone buffer, 2 mM EDTA, pH 8.6, using a 1% agarose gel.(2) But realising the deer had not been shot, he called a vet who confirmed it had been put down by injection and would contain phenol barbitone drugs, which are dangerous.(3) Fischer produced 5.5 - diethylbarbituric acid , a hypnotic and sedative known by the trade names Barbital, Veronal, and Dorminal.